Table 1 : Unfolding and targeting thermodynamic profiles.


    Transition TM 
(°C)
ΔH°
(kcal/mol)
TDS
(kcal/mol)
ΔG°(5)  
(kcal/mol)

PsK-5   -- 52.8 60.1 51.6 8.5
CS-5   -- 47.9 38.4 33.3 5.1
PsKDup-5   -- 49.6 135 116 18.7

PsK-9   -- 59.3 87.5 73.3 14.2
CS-9   -- 48.6 48.5 41.9 6.6
PsKDup-9   1st 47.7 59.2 51.3 7.9
    2nd 58.3 102 85.6 16.4
    Total -- 161 137 24.3

    DSC ITC

    ΔHHC
(kcal/mol)
ΔG°HC 
(kcal/mol)
SHC 
(kcal/mol)
ΔHITC
(kcal/mol)
ΔG°ITC
 (kcal/mol)

PsKDup-5   -36.5 -5.1 -31.4 -28.2 -3.9
PsKDup-9   -25.0 -3.5 -21.7 -23.7 -3.2

All experiments were done in 10 mM sodium phosphate buffer and 100 mM NaCl at pH 7.0.
Experimental errors are as follows: TM (±0.5°C), ΔH (±5%), TΔS (±5%), ΔG°(5) (±7%),
ΔHITC (±5%), ΔG°ITC (± 7%), ΔHHC (±10%), TΔSHC (±10%), ΔG°HC (±14%). Thermodynamic
profiles for the unfolding of the putative structures and their targeting single strands are included.

Marky et al.Biochemical Compounds  2014 2:3DOI : 10.7243/2052-9341-2-3