Figure 8 : RP-HPLC chromatograms showing the blockade of trypsin mediated cleavage of hTTR peptide P1 [41WEPFASGK48↓TSESGELHGLTT60] by NN’-dimethyl formamide (100 μM).

Bottom panel shows the RP-HPLC chromatogram of the peptide digest for 7 minute period. It shows three peaks for the undigested peptide (UP), the C-terminal (CT) and N-terminal (NT) cleaved fragments. The top panel shows the RP-HPLC chromatogram of the same digest in the presence of DMF. It shows only one peak corresponding to the undigested peptide confirming the complete inhibition of cleavage by DMF.

Basak et al.Biochemical Compounds  2016 4:5DOI : 10.7243/2052-9341-4-5