Figure 9 : Protease activity of trypsin following incubation with heparin at various concentrations using the fluorogenic substrate Boc-RVRR-MCA (100 μM).

(A) Bar graph showing the end point fluorescence readings and (B) Progress curves showing slopes for lines in the order from top to the bottom in the absence and presence of heparin solution (1mM) in various amounts as indicated. From top: Trypsin+2.5μl heparin solution; Trypsin+5μl heparin solution; Control Trypsin alone and Trypsin+10μl heparin solution. Slope values are in arbitrary units.

Basak et al.Biochemical Compounds  2016 4:5DOI : 10.7243/2052-9341-4-5