2. Assistant Professor of Medicine, Massachusetts General Hospital Cardiovascular Research Center, 149 13th Street, Charlestown, MA 02129, USA.
3. Siberian State Medical University, 2 Moskovsky Trakt, 634050, Tomsk, Russia.
4. Department of Theoretical and Experimental Physics, National Research Tomsk Polytechnic University, 30 Lenin Avenue, 634050, Tomsk, Russia.
5. Research Scientist, Institute of Physics and Technology, National Research Polytechnic University, 30 Lenin Avenue, 634050, Tomsk, Russia; Federal State Budgetary Scientific Institution "Research Institute for Cardiology", 111a Kievskaya Street, 634012, Tomsk, Russia.
6. Head of Biochemistry Department, Siberian State Medical University, 2 Moskovsky Trakt, 634050, Tomsk, Russia.
Aim: The purpose of the study was to characterize transdermal delivery of xenon through rat skin from a lipophilic solution and from water.
Methods: Sections of skin were obtained from adult rats (n=12) and were placed into static Franz diffusion cells for 24 h. Xenon diffusion coefficients were determined for diffusion from a lipophilic solution (n=6) and from water (n=6) to phosphate buffered saline (PBS) through skin and for diffusion from a lipophilic solution to PBS through a phase boundary in the absence of skin (n=6).
Results: Xenon flux (JXe) through skin from the lipophilic solution was 0.036 mg/hour×cm2 and permeability coefficient (Kp) was 0.003 cm/h; JXe through skin from water was 0.029 mg/h×cm2 and the Kp was 0.002 cm/h. Total time for xenon diffusion through skin from lipophilic solution and from water was ~2 h.
Conclusion: The study presents the first characterization of xenon diffusion through rat skin from multiphase solutions to PBS. These data may be useful for the development of xenon-rich pharmaceutical products for external use.
Keywords: Xenon, rat skin, transdermal delivery, franz diffusion cell