Figure 4 : Caspase-3 and N-cadherin immunostaining in the stable atherosclerotic plaques.
(A) Fibrous plaque with none caspase-3 immunostaining, neither in the extracellular matrix nor in the cells present here.
(B) Anti-caspase-3 antibody negative control. (C) N-cadherin immunostaining in the extracellular matrix and in VSMCs,
particularly in the cytoplasm (insert), of the stable plaques. (D) anti-N-cadherin antibody negative control. (E) Graph.
A bar chart representing a comparison of the % caspase-3 and N-cadherin positive area in stable atherosclerotic plaques
(n. 58), expressed by % positive, dark brown pixels of the analyzed fields. Data are presented as mean±SD. *p<0,05.
A and B, original magnification 10x; scale bars:50 μm. C and D, original magnification 20x; scale bars:10 μm. Insert in
C, original magnification 40x; scale bar:10 μm.