Figure 1 : Adipose-derived stem cells isolation and culture.

Lipoaspirate were washed with phosphate-buffer saline and treated with collagenase type I for 30 min at
37°C. After stopping the enzyme activity and centrifuge, the cellular pellet was re-suspended in appropriate
culture medium and was cultured in a 37°C humidified incubator with a 5% CO2 environment.

Nazem Ghasemi Journal of Histology & Histopathology  2015 2:8DOI : 10.7243/2055-091X-2-8