Figure 5 : Both images (a and c) are derived from ablation trials with the same setting and conditions.

Panel a: Fiji-softwareprocessed immunohistological image of an ablated pulmonary vein of pig 10. The arrow indicates an ablation lesion of approximately 2.38 mm, L =lumen, scalebar=1mm.
Panel b: detail of panel a, indicated by the box, in which the loss of staining is visualized, L=lumen, scalebar= 0.5mm.
Panel c: Fiji-software-processed immunohistological image of an ablated pulmonary vein of pig 11. The black arrows indicate two ablation spots (0.6mm and 1mm) in myocardial layer 1. The white arrows indicate the fat layer between myocardial layer 1 and 2, L=lumen, scalebar=1mm.
Panel d: detail of panel c, indicated by the box, in which the lighter staining visualizes the transition of normal tissue to ablated tissue, L=lumen, scalebar=0.5mm.

Vandecasteele et al.Journal of Histology & Histopathology  2017 4:14DOI : 10.7243/2055-091X-4-14