Glycol methacrylate (GMA, a resin also called 2-hydroxyethyl methacrylate) embedded sections are useful for quantitative (morphometric or stereological) study of histological structures, but detachment of sections (especially thick ones) from microscope slides is common during staining. We found that heating of the sections at a high temperature could prevent the section loss. To confirm our finding, we designed and conducted a series of experiments, comparing the numbers of sections detached after different treatments such as different temperatures (90-290°C) of heating (on hotplate for 30 minutes), different slides (conventional and adhesive), and different thicknesses (10 and 20 μm) of sections. We showed that heating at 115-140°C prevented the GMA sections from detaching, with the light microscopic image unaffected.
Keywords: Detachment of sections, glycol methacrylate, histological structures, thickness of sections