journal of Histology & Histopathology

Journal of Histology & Histopathology

ISSN 2055-091X
Methodology

Detachment of methacrylate-embedded sections from microscope slides can be prevented by heating on hotplate

Yu Xiang and Zheng-Wei Yang*

*Correspondence: Zheng-Wei Yang zwyang@nsmc.edu.cn

Author Affiliations

Morphometric Research Laboratory, North Sichuan Medical College, 234 Fujiang Road, Nanchong, Sichuan 637007, China.

Abstract

Glycol methacrylate (GMA, a resin also called 2-hydroxyethyl methacrylate) embedded sections are useful for quantitative (morphometric or stereological) study of histological structures, but detachment of sections (especially thick ones) from microscope slides is common during staining. We found that heating of the sections at a high temperature could prevent the section loss. To confirm our finding, we designed and conducted a series of experiments, comparing the numbers of sections detached after different treatments such as different temperatures (90-290°C) of heating (on hotplate for 30 minutes), different slides (conventional and adhesive), and different thicknesses (10 and 20 μm) of sections. We showed that heating at 115-140°C prevented the GMA sections from detaching, with the light microscopic image unaffected.

Keywords: Detachment of sections, glycol methacrylate, histological structures, thickness of sections

ISSN 2055-091X
Volume 1
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