journal of Histology & Histopathology

Journal of Histology & Histopathology

ISSN 2055-091X
Methodology

Microwave-accelerated processing of coral tissue histology

Carlos Toledo-Hernández1†*, Irma I Torres-Vázquez2† and José L Serrano-Vélez2

*Correspondence: Carlos Toledo-Hernández c_toledo_hernandez@yahoo.com

†These authors contributed equally to this work.

1. Department of Biology, University of Puerto Rico-Río Piedras, San Juan, Puerto Rico.

Author Affiliations

2. Biological Imaging Group (BIG), University of Puerto Rico-Río Piedras, San Juan, Puerto Rico.

Abstract

Background: Microwave technology has revolutionized histological processing by reducing processing time and improving tissue integrity. We describe the first microwave-accelerated tissue processing procedure for healthy and diseased tissue fragments of two species of corals: the gorgonian Gorgonia ventalina and the scleractinian Acropora cervicornis.

Methods: Fourteen tissue samples from sea fans (eleven healthy and three diseased), and one tissue sample from a healthy A. cervicornis, were decalcified and processed using microwaves. Histological slices were stained using hematoxylin and eosin and immunostained using an antibody against Aspergillus.

Results: By using microwave technology, the decalcification time, as well as the steps and time for tissue processing were significantly reduced while maintaining the integrity of the tissue.

Conclusions: This technique accelerates the chemical processing of coral tissue, while providing high quality and optimal resolution of histological sections.

Keywords: Histology, microwave, tissue processing, coral diseases

ISSN 2055-091X
Volume 1
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