Background: Male infertility is a private problem. There was no ideal medication for treatment of the infertile males. PDE5 inhibitors are line of management for male infertility. Tadalafil is efficient and selective PDE5 Inhibitor. It is known to cause less visual manifistations in contrast to the other drugs.
Aim of the work: This study was designed to evaluate the effect of tadalafil on the histologicsl structure and ultrastructure of adult male albino rats testis.
Material and methods: Twenty-four adult male albino rats were divided into 4groups; control and 3treated groups (T1,T2,T3). Tadalafil was given orally to the treated groups in 3 different doses for 30 days. At the end of the experiment, test is was dissected for histological and electron microscopic study.
Results: Light microscopic examination of T1 revealed normal structure of tubules with intercellular vacuoles. T2 showed thin irregular thin basement membrane, more vacuoles and congested blood vessels. T3 showed acidophilic exudate in the lumen of the tubules with degenerated germinal cells. The electron microscopic examination of T1 showed normal germinal cells structure with some dilated mitochondria. T2 revealed irregular nuclear membrane of both Sertoli and spermatogonia cells. T3 showed interrupted inter-Sertoli junctional specialization, irregular nuclear membrane in sertoli and Leydig cells. Round spermatid showed loss of acrosomal cap.
Conclusion: It can be concluded that, long-term daily use of tadalafil can lead to noticeable pathological effect in the testis which may be implicated in male fertility.
Keywords: Tadalafil, testis, sertoli cells, seminefrous tubules, leydig cells