Background: Oral mucositis (OM) is mucosal barrier injury. It is commonly caused by 5 Flurouracil (5-FU). Glucosamine (GlcN) has many beneficial therapeutic effects. Platelets rich plasma (PRP) are a promising line for curing ofvarious clinical injuries.
Objective: The aim of the study was to evaluate the effect of glucosamine and PRP on experimentally induced oral mucositis in adult male rats.
Materials and methods: 40 adult male rats divided evenly into four groups. Group I (Control). Group II (Mucositis): rats injected one time intraperitoneally with 5-FU(150 mg/kg body weight).Group (III) (Mucositis treated with glucosamine): rats injected intraperitoneally with glucosamine (1000mg/kg body weight) following induction of oral mucositis for 3weeks. Group IV (Mucositis treated with PRP) rats injected with PRP(1ml/rat/day) locally into buccal mucosa following induction of oral mucositis for 3weeks. Buccal mucosa samples prepared and examined by using histological and immunohistochemical techniques.
Results: Group II showed apparent diminish in the thickness of the epithelium with separation of keratin, hemorrhage and perinuclear vacuolization. There were areas of degeneration, intense subepithelial mononuclear infiltration and cell apoptosis (highly expressed caspase-3). A significant reduction of Periodic acid Schiff (PAS) positive reaction and anti-proliferating cell nuclear antigen (PCNA) positive nuclei (P<0.01). Group III&IV showed improvement of the histological and immunohistochemical changes described before.
Conclusion: Glucosamine and PRP provide a promising treatment for oral mucositis accompanying with chemotherapy. PRP remedy is simple and the most efficient mean as it exhibits more rapid epithelial differentiation and wound healing.
Keywords: Glucosamine, 5-FU, Mucositis, PRP, Caspase -3, PCNA