journal of Histology & Histopathology

Journal of Histology & Histopathology

ISSN 2055-091X
Original Research

Gross examination and Histopathology of Fibropapillomas in Chelonia mydas and Lepidochelys olivacea from Baja California Sur, Mexico

Eduardo Reséndiz1,2,3*, Helena Fernández-Sanz2,3,4, Dulce Sofía Barrientos Torres2,3,4, Jorge Armando Vega-Bravo2 and María Mónica Lara-Uc1,2,3

*Correspondence: Eduardo Reséndiz jresendiz@uabcs.mx

1. Departamento Académico de Ciencias Marinas y Costeras, Universidad Autónoma de Baja California Sur (UABCS). Carretera al Sur KM 5.5., Apartado Postal 19-B, C.P. 23080, La Paz B.C.S. México.

Author Affiliations

2. Proyecto Salud de Tortugas Marinas – UABCS, Carretera al Sur KM 5.5., Apartado Postal 19-B, C.P. 23080, La Paz B.C.S. México.

3. Alianza Keloni A.C. Antonio Rosales 698, col. Centro, C.P. 23000, La Paz B.C.S. México.

4. Posgrado en Ciencias Marinas y Costeras (CIMACO) UABCS, Carretera al Sur KM 5.5., Apartado Postal 19-B, C.P. 23080, La Paz B.C.S. México.

Abstract

Background: Fibropapillomatosis (FP) is an infectious disease considered to be one of the primary causes of mortality for many green turtles stokes around the world, commonly associated with a herpesvirus identified as Chelonid herpesvirus type 5 (ChHV-5). To detect the viral particles associated with the specific lesions of FP, Histopathology, Transmission Electron Microscopy and PCR studies have been used; in this manuscript we described by gross Pathology and Histopathology cutaneous FPs in an eastern Pacific Green Turtle (EPGT) C. mydas from Ojo de Liebre lagoon (LOL) and in an olive ridley turtle L. olivacea from the Gulf of Ulloa (GU) in the Baja California Peninsula.

Methods: During September and December 2016, olive ridley turtles were caught at GU and EPGTs were caught at LOL. A physical examination adapted for sea turtles was carried out and morphometric data was taken. Two tissue samples (2 and 3 cm aprox. respectively) from two turtles that presented neoplasms were recollected; the lesions were completely resected, fixed in formalin and sent to the Marine Botany Laboratory at the Autonomous University of Baja California Sur to be processed with the Histopathology routine technique and were reviewed using a microscope with ×10 and ×40 lenses.

Results: One olive ridley turtle (adult female) caught at GU, presented a FP-like lesion at the right eye, in addition to two FP-like lesions at the ventral part of the right anterior flipper; in LOL, an EPGT (sub adult female), presented a FP-like lesion at the ventral base of the right anterior flipper. The examination of the nodules by light microscopy revealed orthokeratotic hyperkeratosis, epidermal hyperplasia with areas of edema, dermal papillary differentiation, increased fibroblasts in the dermis and mild infiltration of lymphocytes arranged at the perivascular level and at the dermo-epidermal junction.

Conclusions: It is important and necessary to complement this kind of studies with the use of PCR to determine the role of the disease causative agent in the development of the neoplasms. Monitoring along the west coast of Mexico is essential to determine the presence, prevalence and incidence of FP and other diseases.

Keywords: Eastern Pacific green turtle (EPGT), black turtle, olive ridley turtle, neoplasms, fibropapillomatosis

ISSN 2055-091X
Volume 6
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