New method for detection of mutagenicity in oral mucosa the through of micronucleus test
2. Department of biology, Centro Universitário Luterano de Ji-paraná, Rondônia, Brasil.
3. Department of chemistry, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil.
4. Department of Health, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil.
Background: The micronucleus assay using a tissue rapidly dividing, such as human oral mucosa, allows for assessment of damage of genetic material, without the need for a stage of ex vivo cell replication.
Methods: The present study describes a new protocol for the detection of MN in oral mucosa, replacing methanol and giemsa by triarilmetano, xanthene and thiazine.
Results: The blades were well visible with ungrouped cells, having a good view of the cell boundaries.
Conclusions: Good results were obtained with the substitution of methanol and giemsa by triarilmetano, thiazine and xanthene, because of its efficiency in blush nucleic acid, getting a good visualization of cell nuclei and micronucleus, and this methodology it is indicated for mutagenicity analysis of the oral mucosa.
Keywords: Mutagenicity, oral mucosa and micronucleus