HOAJ Biology

HOAJ Biology

ISSN 2050-0874
Methodology

New method for detection of mutagenicity in oral mucosa the through of micronucleus test

Dionatas Ulises de Olivei Meneguetti1*, Francisco da Silva2, Rosa Bosso2, Renato Zan3, and Leandro Ramos4

Correspondence: Dionatas Ulises de Olivei Meneguetti dionatas@icbusp.org

1. Department of biology, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil.


Author Affiliations

2. Department of biology, Centro Universitário Luterano de Ji-paraná, Rondônia, Brasil.

3. Department of chemistry, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil.

4. Department of Health, Faculty of Education and the Environment, Ariquemes, Rondônia, Brazil.

Abstract

Background: The micronucleus assay using a tissue rapidly dividing, such as human oral mucosa, allows for assessment of damage of genetic material, without the need for a stage of ex vivo cell replication.

Methods: The present study describes a new protocol for the detection of MN in oral mucosa, replacing methanol and giemsa by triarilmetano, xanthene and thiazine.

Results: The blades were well visible with ungrouped cells, having a good view of the cell boundaries.

Conclusions: Good results were obtained with the substitution of methanol and giemsa by triarilmetano, thiazine and xanthene, because of its efficiency in blush nucleic acid, getting a good visualization of cell nuclei and micronucleus, and this methodology it is indicated for mutagenicity analysis of the oral mucosa.

Keywords: Mutagenicity, oral mucosa and micronucleus

ISSN 2050-0874
Volume 1
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