BALB/c mice elicited specific cell-mediated immunity after transplantation with CT26 cells stably expressing human telomerase reverse transcriptase (CT26(hTERT#10)). Restingmemory T cells were reactivated in vitro by CT26(hTERT#10) cells and PTD-J-hTERTepi recombinant proteins, in which the polypeptide hTERTepi comprised TH and TC epitopes, as indicated by interferon γ (IFNγ) secretion. Preand post-injections of PTD-J-hTERTepi with in vitro expanded and differentiated dendritic cells reduced CT26(hTERT#10) tumor size. Moreover, Hsp72 activated numerous splenocytes to perform IFNγ secretion and further reduced tumor sizes.
Keywords: PTD-J domain, hTERT, epitopes, cellular immunity