Research Journal of Infectious Diseases

Research Journal of Infectious Diseases

ISSN 2052-5958
Original Research

Biological, Serological and Molecular Characterization of Papper MildMottle Virus Isolated from Weast Region of Kingdom of Saudi Arabia

Afaf S. Alwabli1,2*, Eman A.H. Khattab2,4 and Azza G. Farag3,4

*Correspondence: Afaf S. Alwabli aalwabili@stu.kau.edu.sa (or) afafalwabli@yahoo.com

1. Biology Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Author Affiliations

2. Biology Department, Faculty of Science, Taif University 888, Taif, Saudi Arabia.

3. Biotechnology Departments, Faculty of Science, Taif University, Taif, Saudi Arabia.

4. Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agriculture Res. Center (ARC), Giza, Egypt.

Abstract

In this paper, The virus was identified based on host range, symptomatology, and stability in crude extracts, modes of transmission, serological reaction, inclusion bodies, electron microscopy and molecular biology. Study ofthe host range which inoculated different plant species belonging to twelve families revealed that the reactions different hosts. Data concerning stability of virus in crude sap were TIB95C0,DEP10-7-10-8 and LIV 28 days. Modes of transmission showed that PMMoV transmitted mechanically and by infected seeds but cannot be transmitted byany of the tested aphid species.A one-step reverse-transcription polymerase chain reaction (RT-PCR) assay was used for the detection and identification of the isolated virus from nucleic acid extracts of infected pepper plants .Using specific oligonucleotide primer PMMoV-F/ PMMoV-R, which amplified the coat protein (CP) gene for detection of Pepper mild mottle virus (PMMoV). A major product of approximately 387bp PMMoV-CP gene was produced. DNA-DNA hybridization assays of viral genome have been used for detection of the present virus isolate using specific cDNA probe prepared for PMMoV. A one-step reverse-transcription polymerase chain reaction (RT-PCR) assay was used for the detection and identification of the isolated Pepper mild mottle virus (PMMoV) from nucleic acid extracts of infected pepper plants.Using specific oligonucleotide primer PMMoV-F/PMMoV-R, which amplified the coat protein (CP) gene for detection of Pepper mild mottle virus (PMMoV). A major product of approximately 387bp PMMoV-CP gene was produced. A high reliable sensitive immunocapture reverse transcriptionpolymerase chain reaction (IC-RT-PCR) technique was applied for detection of PMMoV in which the in infected pepper plant. PCR fragment of correct size 387bpwas amplified with primer specific coat protein gene. DNA-DNA hybridization assays of viral genome have been used for detection of the present virus isolate using specific cDNA probe prepared for PMMoV. This study of PMMoVis carried out for the first time in Taif Saudia Arabia.

Keywords: Pepper, mild mottle virus, antibodies, purification, UV-absorption spectrum

ISSN 2052-5958
Volume 5
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