Journal of Anesthesiology & Clinical Science

Journal of Anesthesiology & Clinical Science

ISSN 2049-9752
Original Research

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Tânia Kawasaki de Araujo1, Roseane Lopes da Silva-Grecco1, Flora Margarida Barra Bisinotto2, Nilson Camargos Roso2, Cristina Wide Pissetti1, Ricardo Manoel da Cruz1 and Marly Aparecida Spadotto Balarin1*

*Correspondence: Marly Aparecida Spadotto Balarin balarin@mednet.com.br

1. Genetic Department, Institute of Biological and Natural Sciences, Federal University of the Triângulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil.


Author Affiliations

2. Anesthesiology Department, Institute of Health Science, Federal University of Triângulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil.

Abstract

Exposure to certain chemical agents causes damage to the genetic material. There is controversy about the genotoxic and/or mutagenic effect caused by acute or chronic anesthetics exposure. The aim of this research was to assess and compare the frequency of micronuclei (MN) in professionals working in operating rooms and other hospital areas. The genotoxic and cytotoxic effects were evaluated in lymphocytes using the micronucleus test and the proliferative index, respectively. We examined peripheral blood lymphocyte cultures from 30 operating room professionals (exposed group) and 30 professionals non-exposed in other hospital areas of the same hospital (control group). There was no statistically significant difference in proliferative index between the groups. Nevertheless, there was an increase in MN frequency in binucleated (BN) cells in the exposed group (p=0.0003) compared to the control group. Moreover, there was a statistically significant difference between genders (p=0.0187), and the frequency of MN in BN cells from women was higher than in men. Therefore, gender influenced the frequency of MN. The age and period of working time in an operating room influenced the MN frequency only in women professionals. Thereby, there was a genotoxic effect in occupationally exposed professionals, and the micronucleus test could be used in the biomonitoring of human populations for evaluating the risk of developing cancer.

Keywords: Anesthetics, micronuclei test, genotoxic, occupational exposure

ISSN 2049-9752
Volume 2
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