Table 1 : Characteristics of 20 EST-SSR loci.


Marker name Repeat motif Forward primer
sequence (5’-3’)
Reverse primer
sequence (5’-3’)
Expected
size (bp)
Stuteringa Annealing temperature°C

LT002 (GCA)8 CCTACCACCAGCA TCTCGTCGCTGAAGAT 189 N 59
    ATACCTA ATG      
LT015 (CCGAAC)5 TCCGTTATCTCTCT CTAGACAGGTGCTCGG 110 N 59
    CCAAAA ATAC      
LT021 (TTC)8 CAAATACCATTGC ACGCATCCTCTTCCAC 180 N 57
    ACCTTGT TAC      
LT086 (CTT)10 AAGACAGGACTTT GAACGAACCTAACCA 274 N 55
    CCACTGA AATGA      
LT096 (CT)20 TGCAACCTAACAA TGAAAAGCAACCAAG 272 N 55
    GATGTGT TTACC      
LT131 (AC)22 GCAGCATCTCCTC TTGCAGTTGAGCTATT 240 Y 55
    ATATTCT GTTG      
LT157 (TTC)6 AGTTGCCCTTTAGC GCCACAGAGTTTTGGA 222 Y 55
    TTCTTT AGTA      
LTCU19 (AG)10 GTGGATTGCAAAG AAAACAAAAGCAAGC 183 N 57
    GCAGAGT AAGCC      
LTCU40 (ATG)8 TTGCGTAAATGCA GAAGCCtaTGCAAGAT 181 Y 55
    TCCAAAA GCAA      
LTCU51 (CT)18 ATCACCATCTTCCT AAACCATTCCAACCAT 198 N 55
    CATCGC CCAA      
LTCU53 (TG)14 CGGATCTTTCTCTT AAGAAGATTGCAGAG 223 N 55
    TCCATCC GCAGAA      
LTCU125 (TC)8 CGAAAGACATTCC CCATTACAATCCACAG 205 N 55
    CATCACA CCAA      
LTCU139 (TCT)10 GAATAACCGCTCT AAGCCAAGTGGCAAA 164 Y 55
    TTTGGGA GAAGA      
LTCU142 (AAT)8 TGGTGCATATGGG TATTCCCCCAGCTTCT 171 Y 55
    CTTAGAA CCTT      
LTCU143 (TG)13 AAAAATGCTAATC TATCCAACCGATCACC 160 N 55
    CAATAACTTTCG CATT      
LTCU145 (GA)18 TTGAAGTCCAGAT GCCTAGGGaGATGtTTT 157 N 55
    TGATTGATTG TGG      
LTCU150 (TC)10 TCTTCAAACCAAG GCACTACATCCCTTTTc 167 N 55
    GCTGTTG CCA      
LTCU151 (TC)11 TGAGGTGACTTTG GACCCgaGCTGTAAAA 189 N 55
    GCTTTTG TGGA      
LTCU152 (CA)17 CATCCAAATGCAG ATTCCCACTCGGTTGA 177 N 55
    CAGAAAT ACAC      
LTCU154 (CT)10 GATGAAGGAGAAT CCAGCCAAGAAAGAA 156 N 55
    TCTATATTTTCTGA AATGG      

aY:Yes; N:No.

Liang et al.Journal of Plant Science and Molecular Breeding  2015 4:1DOI : 10.7243/2050-2389-4-1