Journal of Plant Science & Molecular Breeding

Journal of Plant Science & Molecular Breeding

ISSN 2050-2389
Original Research

First products of DNA marker-assisted selection in sorghum released for cultivation by farmers in sub-saharan Africa

Abdalla Mohamed1*, Rasha Ali2, Osama Elhassan2, Elnayer Suliman2, Charles Mugoya3, Clet W. Masiga3, Adil Elhusien4 and Charles T. Hash5

*Correspondence: Abdalla Mohamed A.H.Moahmed@cgiar.org

1. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Addis Ababa-Ethiopia.

Author Affiliations

2. Agricultural Research Corporation (ARC), Wad Medani, Sudan.

3. Agro-biodiversity and Biotechnology Program (AGROBIO), Association for Strengthening Agricultural Research in East and Central Africa (ASARECA), Entebbe, Uganda.

4. Department of Botany, Faculty of Science, University of Khartoum, Khartoum, Sudan.

5. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh, India.

Abstract

Striga hermonthica (Del.) Benth. is the major biotic constraint to sorghum production. Its control is difficult and can only be achieved through integrated management strategies that depend mainly on host plant resistance and enhanced soil fertility. However, breeding for resistance is hampered by the complexity of host parasite interactions and lack of reliable screening methods. The invention of molecular markers has enhanced the effectiveness of breeding for resistance. Five genomic regions (QTLs) with linked markers associated with Striga resistance were mapped in sorghum variety N13 by [10]. In this study, to increase the efficiency of marker-assisted selection (MAS), 27 EST-SSR markers in close association with Striga resistance QTLs were also identified and mapped. Populations of backcross (BC3S4) derived from N13 (Striga resistant) X three farmer preferred sorghum cultivars: Tabat, Wad Ahmed and AG-8 (Striga susceptible) were generated. Thirty-one lines (BC3S4) with confirmed Striga field resistance were genotyped with foreground and background selection makers. Twenty resistant lines, with two or more major QTLs were selected for regional evaluation. Of these 10 lines were selected and advanced for multilocation testing, together with Wad Ahmed, Tabat, AG-8, N13, SRN39 and IS9830 as checks. Standard variety trials were conducted in Striga sick plots over three seasons (2009-2011) in Sudan, Gezira Research Station, Damazine, Sinnar, and Gedarif. Results revealed that four lines (T1BC3S4, AG6BC3S4, AG2BC3S4 and W2BC3S4) were Striga resistant and agronomically superior with yields ranging from 180% to 298% higher relative to their recurrent parents. This Striga resistance coupled with superior attributes of the recurrent parent (including very high yield potentials, high grain quality and drought tolerance) will provide adaptation and stability across a wide range of environments. These are the first products of DNA marker-assisted selection (MAS) in sorghum released for cultivation by farmers in sub-Saharan Africa.

Keywords: Striga hermonthica, molecular markers, quantitative trait loci (QTL), simple sequence repeat (SSR)

ISSN 2050-2389
Volume 3
Abstract Download