Analyte

Retention Time (min)

Linearity and Range

Accuracy

Precision

Limits of Detection /Quantitation

Response range   (µg/ml)

R2

Recovery          (% ± S.D)*

Intra day Precision §

(%)

Interday Precision§§  (%)

LOD         (µgml-1)

LOQ        (µgml-1)

C

2.20 ± 0.02

0.5-8.0

0.9999 ± 0.0012

100.71± 1.85

0.77

2.02

0.18

0.50

T

5.70 ± 0.01

0.5-8.0

0.9997± 0.0015

102.23± 4.86

0.63

2.00

0.02

0.07

L

7.90 ± 0.01

1.0- 50.0

0.9997± 0.0020

100.15± 2.09

0.03

2.12

0.26

0.79

Z

12.80 ± 0.01

1.0-80.0

0.9998± 0.0006

100.25± 3.06

0.58

2.55

0.07

0.22

N

15.70 ± 0.00

1.0-80.0

0.9998± 0.0006

103.00± 5.05

0.46

2.49

0.21

0.65

Table 1. Summary of the validation parameters

* n = 9; §: No significant differences between the different concentration levels, p > 0.05;   §§: no significant differences between the days, p >0.05

Christianah Adeyeye et al.Journal of Pharmaceutical Technology and Drug Research  2012 1:4DOI : 10.7243/2050-120X-1-4