Figure 4 : Effects of dose and treatment time for treatment with a combination of Tam and Dox on expression levels of cell cycle regulatory proteins in BT483 cells.


BT483 cells were treated with DMSO alone (CON), 4μM Tam, 4μM Tam plus varying concentrations of Dox, for 48h (A), or 4μM Tam plus 0.5μM Dox for the indicated times (24h,48h) (B). Total cell lysates were prepared and western blot analysis was performed.β-Actin was used as an internal control, to ensure equivalent protein loading. (C) BT483 cells were treated with DMSO alone (CON), 4μM Tam, 4μM Tam, plus various concentrations of Dox for 48h. Total RNA was isolated and the mRNA expressionwas analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Huang et al.Journal of Pharmaceutical Technology and Drug Research  2013 2:12DOI : 10.7243/2050-120X-2-12