Figure 6 : Transfection efficiency of methacrylate-based polyplexes in HeLa cells in the presence and absence of serum.

Nanoassemblies were formed in PBS or SFM with 1 μg of pAcGFP1-C1 using polymer compositions equivalent to N/ P=1, 2 and 4,
respectively. HeLa cells were incubated for 4 hrs in the presence and absence of 10% FBS with polyplexes fabricated with
pHPMA-b-pDMAEMA and ERL. TurboFect™/ pDNA polyplexes prepared according to manufacturer’s instructions were used as positive
control. GFP-expressing cells were identified 48 hrs post-treatment by confocal microscopy. Transfection efficiency for each polymer
was quantified by determining the percentage of GFP-positive cells in three random sections. Data are shown as mean±SD (n=3).

Pauletti et al.Journal of Pharmaceutical Technology and Drug Research  2014 3:2DOI : 10.7243/2050-120X-3-2