Journal of Pharmaceutical Technology & Drug Research

Journal of Pharmaceutical Technology &
Drug Research

ISSN 2050-120X
Original Research

Effects of jianwei qiweibaizhusan on the intestinal microorganisms and enzyme activities

GuangXian Cai, Ao Zeng, NenQun Xiao, SaiNan Zhou, KangXiao Guo and ZhouJin Tan*

*Correspondence: ZhouJin Tan tanzhjin@sohu.com

Author Affiliations:

Hunan University of Traditional Chinese Medicine, Changsha, Hunan province, 410208, PRC.


Abstract

Chinese medicine prescriptions are composed of a variety of Chinese herbal medicine in a compatible format. Because of the complexity of Chinese medicine prescription, we could study it more precisely by minus one herbal medicine of the prescriptions. To study the effects of Jianwei Qiweibaizhusan, Sijunzitang and Qiweibaizhusan on intestinal microorganisms and enzyme activities, and to confirm the scientific property of prescription compatibility on Qiweibaizhusan, microbial culturing methods and enzymological methods were used to determine the number of microbes and enzyme activities in gastrointestinal tract. 70 Kuming mice were randomly divided into 7 groups: the control group, the antibiotics treated group, the minus agastache group, the minus costustoot group, the minus kudzuvine root group, Sijunzitang group and Qiweibaizhusan group. The control group was treated with boiled water, the antibiotics group was treated with antibiotics mixture composed of gentamycin sulfate and cefradine, all other groups were treated with antibiotics mixture and Chinese medicines. After three days of treatment with Chinese medicine, the intestinal microbes and enzyme activities of intestinal contents were measured. The results showed that the color of mice feces turned from black to yellow, the diarrhea mice treated by the minus kudzuvine root group and Qiweibaizhusan group were cured. Compared to the antibiotics group, the amount of bacteria from the minus kudzuvine root group and Qiweibaizhusan group showed significant reduction (P<0.05). Compared to the control group, the amounts of Bifidobacterium spp. and Lactobacillus spp. from each treated group significantly increased (P<0.05).Compared to the control group the amounts of bacteria, Bifidobacterium spp. and Lactobacillus spp. from the antibiotics treated group, significantly reduced (P<0.05), but the amount of fungi was significantly increased (P<0.05).Compared to the antibiotics group, the amount of fungi from each Chinese medicine treated group significantly increased (P<0.05). Compared to the control group, intestinal enzyme activity from every treated group significantly reduced (P<0.05). Compared to the antibiotics treated model group, protease and amylase activities from each every Chinese medicine treated group were significantly higher (P<0.05). Xylanase activities of the minus agastache group and Qiweibaizhusan group were significantly lower than that of antibiotics treated group, the minus agastache group and Sijunzitang group (P<0.05). The ingredients of tonifying qi which exist mainly in Sijunzitang, Ginseng, Rhizoma Atractylodis and Poria cocos, are the key Chinese medicine for the regulation of intestinal physiological flora. Agastache and costustoot played important roles on anti-diarrhea by inhibiting the growth of bacteria and the xylanase activity, and kudzuvine root had the function on improving the amylase activity.

Keywords: Qiweibaizhusan, sijunzitang, intestinal microorganisms, enzyme activity

ISSN 2050-120X
Volume 2
Abstract Download