Figure 1 : Detection of Intervening sequences (IVSs) in rrn loci by PCR.


PCR products which were obtained using various bacterial strains were electrophoresed using 1.5% agarose gel in 0.5x Tris-borate EDTA buffer. The rrl gene may include IVSs at helix 25 (odd numbered lanes) and/or at helix-45 (even numbered lanes). Lower band in each lane  (~730 bp) represents PCR product from rrl gene which has no IVSs, while slower migrating (~833 bp) band indicates presence of IVSs. Two flanking lanes contain DNA molecular weight ladder with indicated bp sizes). DNA templates isolated from various strains, SARB-43 (lanes 1, 2), SARB-45 (lanes 3, 4), SARB-46 (lanes 5, 6), SARB-47 (lanes 7, 8), SARB-30 (lanes 9, 10), SARB-40 (lanes 11, 12), SARB-42 (lanes 13, 14), serovar Typhimurium SL1344 (lanes 15, 16), and E. albertii USDA 181 (lanes 17, 18).

Bhagwat et al.Microbiology Discovery  2013 1:10DOI : 10.7243/2052-6180-1-10