Figure 2 : Effect of kinase blockade on cell migration in the presence of CPT.

(A) Pretreatment with an inhibitor of PI3K (LY; 1μM; 30 min) with/without CPT (10μM) for 6 hours (n=6).
(B) Pretreatment with inhibitors of Rac (EHT; 1μM; 30 min), MAPK (PD; 10μM; 30 min) or PI3K (LY; 1μM; 30 min)
with/without CPT (10μM) for 16 hours (n=16).
(C) Pretreatment with EHT (1μM; 30 min) with/without CPT (10μM) for 22 hours (n=6).
(D) Pretreatment with inhibitors of PKG (DT2; 10 μM), PKA (PKI; 10μM), or PKC (CALC; 100nM) for 30 min prior to
treatment with CPT (10μM) for 16 hours (n=6).
(E) Pretreatment with CALC (100 nM) for 30 min prior to treatment with CPT (10μM) for 22 hours (n=3).
(F) Pretreatment with DT2 (10μM) for 30 min prior to treatment with CPT (10μM) for 22 hours (n=6). For all
experiments appropriate vehicle controls (CON) were used, and *p<0.05 versus CON.

Tulis et al.Archives of Physiology  2015 2:2DOI : 10.7243/2055-0898-2-2