Figure 3 : Effect of kinase blockade on VASP phosphorylation in the
presence of CPT.

(A) Phosphorylation of VASPSer239 in the presence of CPT. Cells were pretreated
with LY (1μM), CALC (100 nM) or PKI (10μM) for 30 min prior to treatment with
CPT (10μM) for 30 min (n=8).
(B) Phosphorylation of VASPSer157 in the presence of CPT. Cells were pretreated
with LY (1μM), CALC (100nM), DT2 (10μM), PD (10μM) or PKI (10μM) for 30 min
prior to treatment with CPT (10μM) for 30 min (n=8).
(C) Phosphorylation of VASPSer157. Pretreatment with EHT (1μM) for 30 min
prior to treatment with CPT (10μM) for 30 min (n=16). VASP expression was
normalized to α-tubulin expression in each experiment. For all experiments
appropriate vehicle controls (CON) were used, and *p<0.05 versus CON.

Tulis et al.Archives of Physiology  2015 2:2DOI : 10.7243/2055-0898-2-2