Figure 5 : Effect of CPT on cell proliferation.

(A) MTT assay: cells were pretreated with LY (1μM) for 30 min prior to treatment with CPT (10μM)
over a 24 hour period (n=10).
(B) BrdU assay: cells were treated with CPT (10-100μM) for 24 hours (n=8).
(C) BrdU assay: cells were pretreated with LY (1 μM) or EHT (1μM) for 30 min prior to treatment
with CPT (10μM) or vehicle for 24 hours (n=6).
(D) Hemocytometry: cells were pretreated with EHT (1 μM), LY (1μM), PD (10μM) or BnzcAMP
(10μM) for 30 min prior to treatment with CPT (10μM) for 72 hours (n=6). Per the manufacturer’s
instructions absorbance was read at specified wavelengths. Using trypan blue exclusion staining
no differences in cell viability were observed in any treatment group (data not shown). For all
experiments appropriate vehicle controls (CON) were used, and *p<0.05 versus CON.

Tulis et al.Archives of Physiology  2015 2:2DOI : 10.7243/2055-0898-2-2