Table 6 : Stem cell conversions and multi-lined cultures.


Population Case Inoculum Conversion
(early INI)
SARC
during growth
Conversion
by SARC
End of culture

Primary   A ITD cysts A2/P -- -- T
  B ITD cysts A2/P t96 P/S T+S+ATD cysts
Subcultures C P P/T -- -- T
  D P P/S, S/T -- -- T+ATD cysts
  F T+ATD cysts A2/P -- -- T+P
  E S+ATD cysts A2/P -- -- P+T+ATD cysts
  G P+T+ATD cysts A2/P, P/S -- -- P+T+ATD cysts
  H P+T+ATD cysts A2/P, P/T -- -- P+T
  I T+P P/S, S/T -- -- T+ATD cysts
  J T+P P/T -- -- T
  K T -- -- -- T

Depending on the kind of inoculi, OCB niches and oxidative stress cultures end as homogenous, apparently homogenous
and heterogenous populations. Only few populations are homogeneous consisting of a single stem cell type (P or T cells).
Apparently homogenous populations contain usually a dominant T cell subpopulation and a minor (coverted) P cell fraction
respectively S cell fraction producing ATD cysts in the subsequent passage. This explains why some cultures contain cysts
and others not. ATD encystment in cultures was defined in the past as an enigmatic "spontaneous" encystment.

Vladimir F Niculescu Stem Cell Biology and Research  2015 2:2DOI : 10.7243/2054-717X-2-2