Figure 3 : Differentiation of MSCs-Adipogenesis and osteogenesis.

(A-B) MSCs were cultured in adipogenic induction media for 21 days. Adipocytes differentiated from MSCs were stained with Oil Red O Solution. Lipid droplets in adipocytes were clearly observed. Cell nuclei were stained with Hematoxylin solution. (C-D) For osteogenesis, MSCs were differentiated in osteogenic induction media for 14 days. Osteocytes containing calcium deposits were visualized with Alizarin Red Solution. (Scale bars-A and C: 300 μm; B: 100 μm; D: 200 μm).

Sakaguchi et al.Stem Cell Biology and Research  2016 3:3DOI : 10.7243/2054-717X-3-3