Table 1 : Primary antibodies used in this study.


Antibody Species Source Dilution

MSC markers

Fibronectin Rabbit Millipore 1:1,500
Collagen type I Rabbit Millipore 1:500
CD 29 (Integrin beta1) Rabbit Millipore 1:500
CD 51 (Integrin alpha V) Rabbit Dr. Thomas Joos, NMI, University of Tübingen 1:200
CD90 (Thy-1.1) Mouse Dr. Alan F. Williams, University of Oxford 1:200
CD54 (ICAM-1) Mouse Millipore 1:100
CD11b Mouse Millipore 1:100
CD45 Mouse Millipore 1:100
CD14 Mouse Millipore 1:1,000
CD44 (Hermes-1) Rat Developmental Studies Hybridoma Bank (DSHB) 1:200
Mouse IgG Mouse Millipore 1:100
Rabbit IgG Rabbit Millipore 1:500

Proliferation marker

BrdU Rat DAKO Corp. 1:100

Neural markers

Nestin Mouse DSHB 1:200
III β-tubulin (TuJ1) Mouse R&D systems 1:200
MAP2ab Mouse Sigma 1:200
GFAP Mouse Fisher Scientific 1:500
RIP Mouse DSHB 1:1,000

CD: Cluster of differentiation; MAP2ab: Microtubule associated protein 2ab; GFAP: Glial fibrillary acidic protein;
RIP: Receptor interacting protein

Sakaguchi et al.Stem Cell Biology and Research  2016 3:3DOI : 10.7243/2054-717X-3-3