Stem Cell Biology and Research

Stem Cell Biology and Research

ISSN 2054-717X
Original Research

Effect of cytochalasin types on the production of heterozygous parthenogenetic porcine embryos and the isolation of putative parthenogenetic embryonic stem cells

Ivan Vassiliev*, Anders Tsui, Wan Xian Kang, Stephen McIlfatrick and Mark B. Nottle

*Correspondence: Ivan Vassiliev ivan.vassiliev@adelaide.edu.au

Author Affiliations

Reproductive Biotechnology Group, Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Centre for Stem Cell Research, Robinson Institute, University of Adelaide, Adelaide, Australia.

Abstract

Parthenogenetic embryos have been suggested as an alternative source of embryonic stem cells (ESCs). The present study was undertaken to determine the efficiency with which porcine heterozygous parthenotes could be produced using cytochalasin B (CB) or cytochalasin D (CD) and whether parthenogenetic ESCs (pESCs) could be isolated from these. Cleavage rate was lower and fewer embryos developed to the blastocyst stage in the CB group compared with the CD group. The number of primary outgrowths obtained was also lower in the CB compared with the CD group. No primary lines were isolated from embryonal outgrowths in the CB group. In contrast, primary cell lines were derived from these in the CD group. These lines survived vitrification and warming, resulting in established cell lines, which maintained a characteristic ESC morphology and expressed the pluripotent markers Oct4 and Nanog following repeated passaging. Putative pESC lines could also be directly differentiated to cell types representative of all three germ layers.

Keywords: Parthenotes, Porcine, Heterozygosity, Heterozygous ESC

ISSN 2054-717X
Volume 1
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