Table 4 : Cumulative proteoglycan (PG), nitric oxide (NO), prostaglandin E2 (PGE2), and interleukin-6 (IL-6)
release from cartilage into media for experiment 3 when explants were treated with 0 to 25 μg/mL of
eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)alone or in combination
(Table 1).


  100 LA 25 EPA 18.75 EPA,
6.25 DHA
12.5 EPA,
12.5 DHA
6.25 EPA,
18.75 DHA
25 DHA SEM P-value

PG                

Pre IL-1 66.1 62.8 68.2 69.6 65.5 65.1 4.92 0.941
Post IL-1 473.7 427.7 383.4 478.5 386.1 352.5 40.7 0.189

NO                

Pre IL-1 13.5 12.6 12.6 12.0 11.8 12.1 1.4 0.963
Post IL-1 72.7a 59.1b 52.54b 54.7bc 47.72c 28.5d 3.6 <0.001

PGE2                

Pre IL-1 91.0 222.0 292.7 235.3 218.5 376.8 87.44 0.302
Post IL-1 1379.8a 578.2b 471.7b 521.8b 357.1b 641.9b 149.8 0.001

IL-6                

Pre IL-1 25.5 120.5 28.4 145.4 114.0 38.4 57.95 0.529
Post IL-1 6830.8 9870.4 10172 12468 7255.2 6852.9 1768 0.163

NO and PGE2 release exhibited a treatment effect (P<0.01) post interleukin-1 (IL-1) stimulation.
Means with different letters differ (P<0.05) for a given analyte.

Orth et al.Veterinary Medicine and Animal Sciences  2015 3:4DOI : 10.7243/2054-3425-3-4