Table 5 : Cumulative proteoglycan (PG), nitric oxide (NO), prostaglandin E2 (PGE2), and
interleukin-6 (IL-6) release from cartilage into media for experiment 4 when explants
were treated with 12.5 or 25 μg/mL of eicosapentaenoic acid (EPA) and docosahexaenoic
acid (DHA)
(Table 1).


  100 AA 12.5 EPA 25 EPA 12.5 DHA 25 DHA SEM P-value

PG              

Pre IL-1

60.9 64.5 78.8 57.9 61.4 6.0 0.125

Post IL-1

126.6ac 208.3b 230.8b 152.2a 96.6c 16.6 <0.001

NO              

Pre IL-1

6.9 7.0 4.0 7.0 4.1 1.7 0.471

Post IL-1

9.2a 17.4b 21.8c 10.0ad 8.9ad 1.3 <0.001

PGE2              

Pre IL-1

34695a 20240b 11180bc 19880b 8680c 3304 <0.001

Post IL-1

122110a 76420b 30290c 80135b 81670b 7080 <0.001

IL-6              

Pre IL-1

1.8 3.7 1.7 2.3 2.4 1.5 0.884

Post IL-1

585.8a 2287.6b 4136.4c 2309.5b 238.7a 601.1 <0.001

All variables exhibited a treatment effect post interleukin-1 (IL-1) stimulation (P<0.01).
Means with different letters differ (P<0.05) for a given analyte.

Orth et al.Veterinary Medicine and Animal Sciences  2015 3:4DOI : 10.7243/2054-3425-3-4