Figure 2 : SDS-PAGE analysis of recombinant H6-'HepK and H6-DevR.

A: kDa, protein standards (molecular masses in kDa) shown to the left of lane 1. Lane 1, total extracts of E. coli  BL21 (DE3)
bearing pET-14b. Lane 2, total cell extracts of E. coli  BL21 (DE3) bearing pRL2406 induced with IPTG. Lane 3, H6-’HepK
(~20 μg in 10 μl) purified by a cobalt-based affinity column followed by Sephadex G-100 chromatography.
B: Lane 1, total extracts of E. coli  BL21 (DE3) bearing pET-14b. Lane 2: total cell extracts of E. coli  BL21 (DE3) bearing pRL2461
(Zhou & Wolk, 2003) induced with IPTG. Lane 3, H6-DevR (~10 μg in 10 μl) purified by a cobalt-based affinity column followed by
Sephadex G-100 chromatography. The 12% polyacrylamide gel (A) and the 15% polyacrylamide gel (B) were stained with
Coomassie brilliant blue R-250.

Zhou et al.Trends in Bacteriology   2014 1:3DOI : 10.7243/2057-4711-1-3