Table 1 : Thermodynamic Profiles for the Folding of Pseudoknots
and Control Duplexes.


[NaCl] Transition TM (°C) ∆Hcal
(kcal/mol)
∆20
(kcal/mol)
T∆S
(kcal/mol)

CG-PsK

1 M 1st 43.0 -33 -2.4 -30.6
  2nd 76.3 -54 -8.7 -45.3
  Total   -87 -11.1 -75.9

5 M 1st 42.3 -35 -2.5 -32.5
  2nd 75.0 -52 -8.2 -43.8
  Total   -87 -10.7 -76.3

GC-PsK

1 M 1st 42.8 -4 -0.3 -3.7
  2nd 60.2 -20 -2.4 -17.6
  3rd 82.0 -55 -9.6 -45.4
  Total   -79 -12.3 -66.7

5 M 1st 34.7 -7 -0.3 -6.7
  2nd 52.8 -14 -1.4 -12.6
  3rd 76.4 -48 -7.7 -40.3
  Total   -69 -9.4 -59.5

Du-CG

1 M  -- 65.4 -51 -6.3 -44.7
5 M  -- 55.3 -49 -5.7 -43.3

Du-GC

1 M  -- 67.1 -51 -7.1 -43.9
5 M  -- 54.8 -48 -5.1 -42.9

All experiments were done in 10 mM  sodium phosphate buffer with 1 M or 5 M NaCl
at pH 7.0. Experimental errors are as follows: TM (±0.5ºC), ∆Hcal (±5 %),
T∆S (±5%), ∆G° (20) (±7%).

Marky et al.Biochemical Compounds  2017 5:4DOI : 10.7243/2052-9341-5-4