Table 1 : Optical characteristics and spectral parameters of probes binding with blood plasma albumin.


Parameter   ANS K-35 PR ABM

Maxima excitation and emission of fluorescence (nm)  of probes in complex with albumin   370/470 430/515 525/625 470/650
Stokes shift (nm)   100 83 100 180
Quantum yields of fluorescence in complex with albumin (QY)   0.5-0.9 0.12 0.8 0.08
Constant of binding  (х 105М-1)   5.9 1.8 4.7 1.8
Binding sites number per 1 molecule of albumin (human plasma albumin standard)   2-3 2 2 2-3
Parameter of fluorescence activation: ratio of fluorescence intensity of bound (Fb) with albumin and free (Ff) probe   105 18 34 70

Notes and references:
ANS: (8-Anilinonaphthalene-1-sulfonic acid) [2,3,13]
K-35:N-carboxyphenylimide of 4-dimethylamino-1,8-naphthalenedicarbonic acid [2,3]
PR: Pirrone red [2,3]
ABM: Derivative of 3-aminobenzanthrone [1,5]

Kalnina et al.Journal of Autoimmunity and Cell Responses  2014 1:1DOI : 10.7243/2054-989X-1-1