Figure 3 : Localization of GFP-ZYG1 fusion protein, GFP protein and cellulose in the macrocyst-like
structure and macrocyst.

Bright-field photomicrographs and fluorescence photomicrographs of the same fields are shown. Calcofluor staining is
shown in orange and GFP protein is shown in green.
(a) Starved GFP-ZYG1OE cells were developed as described in the legend of Figure 1. After staining with Calcofluor,
a macrocyst-like structure at 25 days of development was photographed. GFP-ZYG1 fusion protein is localized at the
whole central cell mass and the outer layer of the macrocyst wall, co-localizing with Calcofluor staining.
(b) Starved GFPCONT and V12M2 cells were mixed at a ratio of 1:1 at a density of 6x106 cells/dish and developed. After
staining with Calcofluor, a macrocyst at 22 days of development was photographed. GFP protein is localized at a whole
giant cell and the outer layer of a macrocyst wall, co-localizing with Calcofluor staining. Bars: 50 μm.

Amagai et al.Research Journal of Developmental Biology  2014 1:2DOI : 10.7243/2055-4796-1-2