Figure 4 : Formation of globular bodies.

(A) Final structures formed by co-cultures of GFP-ZYG1OE or GFPCONT cells with V12M2 cells. (a) and (b) Starved
GFP-ZYG1OE cells were mixed with V12M2 cells at a ratio of 1:1 at a density of 6x106 cells/dish and developed.
(a) A bright-field photomicrograph of macrocysts with thick macrocyst walls at 5 days of development. Two kinds of
macrocyst wall are observed: one is lined with peripheral cells (arrow), and another with few peripheral cells (arrowhead).
(b) A phase-contrast photomicrograph of globular bodies at 6 days of development.
(c-e) Starved GFPCONT cells were mixed withV12M2 cells at a ratio of 1:1 at a density of 6x106 cells/dish and developed.
(c) A phase-contrast photomicrograph of a macrocyst surrounded with a macrocyst wall (arrow) and globular bodies
(arrowheads) at 7 days of development.
(d) A bright-field photomicrograph of a macrocyst with a clear macrocyst wall at 21 days of development.
(e) A bright-field photomicrograph of a macrocyst with a macrocyst wall filled with cells at 21 days of development.
Bars: 100 μm.
(B) Formation of cellulosic walls in globular bodies. Starved GFP-ZYG1OE and V12M2 cells were developed as described
in A. At 12 days of development, globular bodies were photographed after staining with Calcofluor. Upper panel:
A phase-contrast photomicrograph of globular bodies. They contain endocytes (arrowhead). Lower panel:
a fluorescence photomicrograph corresponding to the upper panel. As the periphery of globular bodies is stained with
Calcofluor (arrows), it is evident that the globular body is surrounded by a thin cellulosic wall. Bars: 50 μm.

Amagai et al.Research Journal of Developmental Biology  2014 1:2DOI : 10.7243/2055-4796-1-2