Figure 5 : The developmental process of globular body formation by GFP-ZYG1OE or GFPCONT cells
co-cultured with V12M2 cells.

a-c Starved GFP-ZYG1OE and V12M2 cells were developed as described in the legend of Figure 4.
(a) A phasecontrast photomicrograph at 1 day of development. Large aggregates (arrowhead) and free-living giant
cells (arrows) are formed, though many cells remain as single cells.
(b) A phasecontrast photomicrograph at 4 days of development. Giant cells filled with granular endocytes are formed
(arrows).
(c) A phase-contrast photomicrograph of globular bodies (arrows) at 6 days of development. d-f Starved GFPCONT and
V12M2 cells were developed as described in the legend of Figure 4.
(d) A phase-contrast photomicrograph at 1 day of development. Cells are forming an aggregate. A free-living giant
cell (arrow) is observed.
(e) A phase-contrast photomicrograph at 5 days of development. Giant cells filled with granular endocytes are formed
(arrows).
(f) A phase-contrast photomicrograph of globular bodies (arrows) at 6 days of development. Bar: 100 μm.

Amagai et al.Research Journal of Developmental Biology  2014 1:2DOI : 10.7243/2055-4796-1-2