Figure 2 : Immunoprecipitation of neurocan isolated from early chick embryos.

Embryos at stages HH5 (head process) to HH8 (5-somites) were precipitated either with monoclonal
IgG directed at neurocan (lane a) or with nonimmune mouse IgG (lane b). Precipitates were analyzed
in 5% slab SDS-PAGE and stained with Coomassie brilliant blue. The anti-neurocan monoclonal antibody
(clone 1F6) recognized a band at molecular mass of approximately 245 kDa (lane a, arrow) not recognized
by the nonimmune mouse IgG and it is likely to correspond to chick neurocan. Numbers to the left refer
to the migration of molecular mass protein markers in kilodaltons.

Zagris et al.Research Journal of Developmental Biology  2014 1:3DOI : 10.7243/2055-4796-1-3