Figure 4 : Neurocan immunolocalization in chick embryo at stage HH13 (19 somites).

Transverse sections at the heart (A) and pharyngeal arch (C) levels. (A) Neurocan immunoreactivity was intense in
myelencephalon (mn), notochord (n), pre-migratory and in migrating neural crest cells (c) underlying the ectoderm and
inserted in the periaortic region and the gut (g) lateral and lower wall, dorsal aorta (d) and common cardinal vein (v), in
dorsal mesocardium (cd) and was strong in myocardium (cm) and endocardium (ce) and in extracellular matrix (em) in
myelencephalon lumen; (B) intense neurocan localization in an organized extracellular matrix in myelencephalon lumen
shown in (A) presented under higher magnification. (C) At the pharyngeal arch level of the same embryo, neurocan
immunoreactivity was intense in myelencephalon (mn), notochord (n), and the premigratory and migratory neural crest cells
(c) underlying the ectoderm (ec) and colonizing the pharynx (px) lateral and lower wall and the pharyngeal (pa) and aortic (a)
arches; (D) distinct neurocan fluorescence in neural crest cells emerging from the myelencephalon as shown in (C) start their
migration as a broad wave presented at higher magnification. (E) Neural crest cells emerging from the myelencephalon and
migrating shown in embryonic section (A) display intense neurocan fluorescence in the main body and filopodial protrusions
presented at higher magnification. am: amnion. Scale bar 50μm in (A,C), 25μm in (D) and 10μm in (B,E).

Zagris et al.Research Journal of Developmental Biology  2014 1:3DOI : 10.7243/2055-4796-1-3