Figure 5 : Neurocan immunolocalization in chick embryo at stage HH17 (29 somites).

Transverse sections at the eye (A), heart (B) and trunk (C) levels. (A,B) Neurocan immunoreactivity was intense
in telencephalon (tn), diencephalon (dn), myelencephalon (mn), neural tube (nt), infundibulum (fn) and Rathke’s
pouch (p), the notochord (n) periphery, the sympathetic neural ganglia (gl), neural crest cells (c) and head
mesenchyme (mh), nasal placode (ns), the lens (ln) and presumptive cornea (cn), neural (rn) and pigmented (rp)
retina, myocardium (cm) and endocardium (ce) of bulbus cordis (cb), intense in myotome (mt) and strong in
sclerotome (sc), intense in dorsal aorta (d) and common cardinal vein (v) and periaortic mesenchyme, in pharynx
(px) and in the splanchnic mesoderm (mp) lining the pharynx, and was distinct in extracellular matrix (em) in
pharynx and brain lumen. (C) At the trunk region, neurocan was intense in the neural tube (nt), notochord (n),
strong in dermamyotome (dt) and distinct in sclerotome (sc), strong in nephrotome (ne) and intense in
presumptive pronephric tubules (pt), strong in lateral somatic mesoderm (ms), intense in blood islands (b) and
strong in endoderm (en). (D) Higher magnification image of the eye from embryonic section neighboring the
embryonic section in (A). as: aortic sac; ec: ectoderm; mp: splanchnic mesoderm. Scale bar 50μm in (A-C) and
25μm in (D).

Zagris et al.Research Journal of Developmental Biology  2014 1:3DOI : 10.7243/2055-4796-1-3