Figure 1 : Schematic representation of the AphVIII cassette used for generation of insertional mutants.

Primers annealing-sites used for amplification of the genomic regions flanking the insertion, both by inverse PCR (1) and
RESDA PCR (2), are inducated. The sequence of these primers is shown in Table I.

León et al.Genomics Discovery  2013 1:2DOI : 10.7243/2052-7993-1-2