ISSN 2052-6210
Original Research

Clinical significance of thin cytology and HPV DNA test in diagnosing cervical disease

Song Ji Rong1, Rajina Shrestha1*, Dipesh Pradhan2, Koun Linka1 and Jasma Mally3

*Correspondence: Rajina Shrestha

1. Department of Obstetrics and Gynecology, Jiamusi University, Jiamusi 154007, Heilongjiang PR China.

Author Affiliations

2. Norman Bethune College of Medicine, Jilin University, Changchun 130021, China.

3. Department of Obstetrics and Gynecology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China.


Objectives: To evaluate the sensitivity and accuracy of the HPV DNA test in conjunction with Thinprep cytology test as a screening method of human papillomavirus (HPV) infection.

Method: In our retrospective study, 158 women with age group 21-70 years having positive thin cytology test were recruited. A computerized search identified patients with ASCUS, LSIL, HSIL in Thinprep test results and high risk HPV DNA testing and cervical biopsy results of these patients.

Results: Out of 158 patients, HPV DNA tests were positive in 52 (32.9%) and negative in 106 (67.1%). High grade CIN and Carcinoma Cervix were commonly associated with ASCUS and HSIL as CIN I, II-III, III, Carcinoma Cervix, were 53.6%, 24.3%, 22.2% and 15% respectively for ASCUS (n=67); and 0%, 63.2%, 77.8%, 75% for HSIL (n=49). HPV DNA positive extremely favors CIN II-III, III and cervical cancer as Human Papilloma Virus DNA test were positive in 22 (78.6%) out of 28 cases of CIN I, 36 (97.5%) out of 37 cases of CIN II-III, 9 (100%) out of 9 cases of CINIII and 4 (100%) out of 4 cases of cervical cancer. There was significant correlation of TCT with HPV, age and CIN (P<0.0001). HPV DNA was common in increasing age group as compared to young age (21-30 years, n=39 [46.2%] vs. 61-70 years, n=4 [100%]).

Conclusion: Combined thin cytology test along with HR HPV DNA test has great value in determining high grade of cervical intraepithelial neoplasia and cervical eoplasia.

Keywords: Human papilloma virus, cervical intraepithelial neoplasia, squamous intraepithelial neoplasia, atypical squamous cells thin cytology test

ISSN 2052-6210
Volume 1
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