Figure 1 : Spleen and Lymph Node.

The frozen sections of spleen showed diffuse positive staining in the sinusoids for LYVE-1 in larger caliber capillaries and positive staining for F-8 in smaller caliber capillaries (A and B) and central arteries and larger blood vessels were strongly stained for F-8 (B). The paraffin sections showed strong staining for LYVE-1 in larger capillaries (C) and double staining showed positive staining in larger capillaries for LYVE-1 but negative for F-8 while central arteries were positive F-8 but not in sinusoidal capillaries (D). The frozen sections of lymph node showed densely packed LYVE-1 positive lymphatic vessels in the sinusoids and F-8 positive blood vessels in medulla and hilar connective tissue (E and F). c: central artery, s: lymphatic sinusoid A, B, E and F: Frozen sections, C and D: Paraffin-embedded sections A, C and E: LYVE-1, B and F: F-8, D: LYVE-1 and F-8 immunostained.

Tomita et al.Journal of Histology & Histopathology  2023 10:1DOI : 10.7243/2055-091X-10-1