Figure 3 : Light microscopy images of intact hind paws (toe (A,B,C), metatarsal (D,E,F), footpad (G,H,I)) fixed (4% PFA) for 20 or 24 hours, and processed (automated) using a 9 or 12 hour protocol.

Sagittal sections (7μm) stained with hematoxylin and eosin. Note the improved preservation of tissue morphology and integrity throughout the hind paw with longer fixation and shorter processing times (B,E,H).

Clark et al.Journal of Histology & Histopathology  2020 7:1DOI : 10.7243/2055-091X-7-1