Figure 7 : Anti-ENT1 labeling on cells growing on Transwell inserts.

Light micrographs (CLSM) images of V19 human vaginal epithelial cells grown as a multi-layer sheet on Transwell filter membranes and labeled with anti-ENT1 antibodies. The antibody labeled non-defined regions on the multilayer sheet of cells. A) The antibody labeled regions around the cell nuclei (DAPI, in blue), but seldom over the nuclear region. Phalloidin label was not extensive throughout the cell layers, and was restricted to regions where there were lower amounts of anti-ENT1 label. B) The anti-ENT1 label was associated with cells and had a punctate appearance. The label was around the cell nuclei but seldom over the nuclear region. In some instances the antibody label was concentrated over perinuclear spots. Phalloidin labeling is green and DAPI (nucleic acids) is blue. Scale Bars=20 μm.

Webster et al.Journal of Histology & Histopathology  2020 7:5DOI : 10.7243/2055-091X-7-5