2. Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, (U.P.), India.
3. Institute of Biomedical Sciences, Bundelkhand University, Kanpur Road, Jhansi 284001, Uttar Pradesh, India.
The aim of this study was to examine and compare the toxic effects of endosulfan and ochratoxin-A (OTA), individually and in combination, in adult male rats with respect to histopathological changes over 30 days of treatment. Adult male Wistar rats were randomly allotted to four groups (individual treatments, combination and control) of 10 rats each and fed OTA @ 4 ppm in feed (Group-I), endosulfan @ 5 mg/kg BW in corn oil by oral gavage (Group-II), and endosulfan with OTA in combination (Group-III) daily for 30 days. Group IV was kept as control and fed toxin free feed. After 30 days of treatment, tissues were collected in 10% buffered formalin for histopathological studies. The hematoxylin and eosin stained paraffin sections showed varying degree of degenerative and necrotic changes in kidneys and liver which were more severe in OTA treated rats in comparison to endosulfan fed group. However, the changes observed in group III rats, which received both OTA and endosulfan were even more pronounced than those observed in rats fed OTA or endosulfan alone. The present findings suggest that OTA and endosulfan had additive effects which may play an important role in pathogenesis.
Keywords: Ochratoxin-A, endosulfan, toxicity, histopathology, liver, kidney