journal of Histology & Histopathology

Journal of Histology & Histopathology

ISSN 2055-091X

Combined toxicity of endosulfan and ochratoxin-A in rats: histopathological changes

Shashi Nandar Kumar1, Banajit Bastia1, Avinash Gopal Telang2, Karam Pal Singh2, Rambir Singh3 and Arun Kumar Jain1*

*Correspondence: Arun Kumar Jain

1. Environmental Toxicology Lab, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi-110029, India.

Author Affiliations

2. Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, (U.P.), India.

3. Institute of Biomedical Sciences, Bundelkhand University, Kanpur Road, Jhansi 284001, Uttar Pradesh, India.


The aim of this study was to examine and compare the toxic effects of endosulfan and ochratoxin-A (OTA), individually and in combination, in adult male rats with respect to histopathological changes over 30 days of treatment. Adult male Wistar rats were randomly allotted to four groups (individual treatments, combination and control) of 10 rats each and fed OTA @ 4 ppm in feed (Group-I), endosulfan @ 5 mg/kg BW in corn oil by oral gavage (Group-II), and endosulfan with OTA in combination (Group-III) daily for 30 days. Group IV was kept as control and fed toxin free feed. After 30 days of treatment, tissues were collected in 10% buffered formalin for histopathological studies. The hematoxylin and eosin stained paraffin sections showed varying degree of degenerative and necrotic changes in kidneys and liver which were more severe in OTA treated rats in comparison to endosulfan fed group. However, the changes observed in group III rats, which received both OTA and endosulfan were even more pronounced than those observed in rats fed OTA or endosulfan alone. The present findings suggest that OTA and endosulfan had additive effects which may play an important role in pathogenesis.

Keywords: Ochratoxin-A, endosulfan, toxicity, histopathology, liver, kidney

ISSN 2055-091X
Volume 2
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