2. Department of andrology, Sexology& STDs, Faculty of Medicine, Beni-suef University, Egypt.
3. Department of andrology, Sexology& STDs, Faculty of Medicine, Cairo University, Egypt.
Background: Many harmful effects have been reported for the abuse of testosterone with supraphysiologic doses on the male genital system.
Objective: To study the possible protective effect of silymarin in testosterone induced testicular changes.
Materials and Methods: Eighty adult male albino rats were randomly divided into four groups; untreated rats (controls), rats treated with silymarin, rats treated with testosterone and rats treated with testosterone and silymarin. Relative testicular weight, serum testosterone and FSH were estimated in addition to histological, histochemical and immunohistochemical assessment.
Results: Serum testosterone was significantly higher in testosterone treated rats compared with the controls and rats treated with testosterone plus silymarin. Serum FSH was significantly lower in testosterone treated rats as well as rats treated with silymarin plus testosterone compared with controls. There was a significant increase in caspase-3 expression between testosterone treated rats and the other studied groups (P<0.001). There was a significant increase in P53 expression between the testosterone treated rats and the control groups (P<0.001 for each), and also between the testosterone treated rats and rats treated with testosterone and silymarin (P=0.004). P53 expression was also significantly different between rats treated with testosterone plus silymarin and the control groups (P=0.012 for each).
Conclusion: Oral concomitant treatment with silymarin was effective in attenuating testosterone- induced testicular damage in adult male albino rats.
Keywords: Testosterone, testis, apoptosis, caspase, P53