2. Department of Biochemistry, Faculty of Veterinary Medicine, University of Ataturk, TR-25240, Erzurum, Turkey.
3. Department of Physiology, Faculty of Veterinary Medicine, University of Ataturk, TR-25240, Erzurum, Turkey.
4. Department of Physiology, Faculty of Veterinary Medicine, University of Kafkas, TR-25240, Kars, Turkey.
Background: Obesity is a worldwide health problem and causes many important illnesses. The current study aimed to investigate the influence of quercetin over apoptosis by inflammatory activity on high-fat dietinduced liver damage.
Material and methods: Totally 18 adult female Sprague-Dawley rats were selected and randomly separated equally into three groups, followed as control, obesity and obesity-quercetin groups. Obesity and obesityquercetin groups were fed a moderately high fat diet for 120 days. After obesity occurred, quercetin dissolved in the corn oil and given obesity-quercetin group at dose of 50 mg/kg by oral gastric way for 15 days. All animals were sacrificed with an anesthesia at the end of the experiment, and venous blood samples were collected from the right ventricle and hepatic markers were evaluated by using commercially available diagnostic kits. In addition, liver sections were analysed by histopathologic and immunohistochemical procedures. Also, we evaluated the expression of Bax and Bcl-2 by immunohistochemistry.
Results: Obesity induced important change (p<0.05) in the most of biochemical parameters, hepatic markers, and Bax immunoreactivity as well. But, the level of Bcl-2 was slightly increased, in contrast, the level of Bax decreased markedly (p<0.05) in the obesity-quercetin group.
Conclusions: Both immunohistochemical evidence and biochemical results showed that application of quercetin decreased the obesity mediated damage to the liver.
Keywords: Apoptosis, liver, obesity, oxidative stress, quercetin