Figure 1 : Inducible expression of mRNA and asRNA in IL-1β- treated hepatocytes.

The expression of mRNA (upper panel) and asRNA (lower panel) in the absence (–) or presence (+) of IL-1β was analyzed by strandspecific RT-PCR. Total RNA from purified hepatocytes treated without or with IL-1β (1 nM) for 4 h was subjected to strand-specific RT-PCR and resolved by 2.5% agarose gel electrophoresis. An oligo(dT) primer and gene-specific sense primers (Table 1) were used in RT to synthesize cDNA to mRNA and asRNA, respectively. The expression of both mRNA and asRNA transcribed from the genes encoding IL-23A, CCL2, CCL20, CX3CL1, and CD69 was analyzed with specific PCR primer sets (Table 1). EF mRNA was used as an internal control for RT-PCR. RT(–) indicates a negative PCR control without RT to evaluate genomic DNA contamination.

Nishizawa et al.  2012 1:10DOI : 10.7243/2050-0874-1-10